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1. | | SOUSA, C. C.; GOMES, S. O.; LOPES, A. C. A.; GOMES, R. L. F.; BRITTO, F. B.; LIMA, P. S. C.; VALENTE, S. E. S. Comparison of methods to isolate DNA from Caesalpinia ferrea. Genetics and Molecular Research, v. 13, n. 2, p. 4486-4493, 2014. Biblioteca(s): Embrapa Meio-Norte. |
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Registro Completo
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
17/12/2014 |
Data da última atualização: |
17/12/2014 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
ALVAREZ-PIZARRO, J. C.; GOMES FILHO, E.; PRISCO, J. T.; GROSSI DE SA, M. F.; OLIVEIRA NETO, O. B. de; FRAGOSO, R. da R. |
Afiliação: |
MARIA FATIMA GROSSI DE SA, CENARGEN; RODRIGO DA ROCHA FRAGOSO, CPAC. |
Título: |
Plasma membrane H+-ATPase in sorghum roots as affected by potassium deficiency and nitrogen sources. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Biologia Plantarum, v. 58, n. 3, p. 507-514, 2014. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: We studied the influence of inorganic nitrogen sources (NO3- or NH4+) and potassium deficiency on expression and activity of plasma membrane (PM) H+-ATPase in sorghum roots. After 15 d of cultivation at 0.2 mM K+, the plants were transferred to solutions lacking K+ for 2 d. Then, K+ depletion assays were performed in the presence or absence of vanadate. Further, PMs from K+-starved roots were extracted and used for the kinetic characterization of ATP hydrolytic activity and the immunodetection of PM H+-ATPase. Two major genes coding PM H+-ATPase (SBA1 and SBA2) were analyzed by real-time PCR. PM H+-ATPase exhibited a higher Vmax and Km in NH4+-fed roots compared with NO3--fed roots. The optimum pH of the enzyme was slightly lower in NO3- -fed roots than in NH4+ -fed roots. The vanadate sensitivity was similar. The expressions of SBA1 and SBA2 were increased in roots grown under NH4+. Concomitantly, an increased content of the enzyme in PM was observed. The initial rate of K+ uptake did not differ between plants grown with NO3- or NH4+, but it was significantly reduced by vanadate in NH4+-grown plants. |
Palavras-Chave: |
Nitrato de Amonia. |
Thesagro: |
Fertilizante nitrogenado; Potássio; Sorghum Bicolor; Sorgo. |
Thesaurus NAL: |
Ammonium nitrate; Nitrate fertilizers; Potassium. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
Marc: |
LEADER 01933naa a2200277 a 4500 001 2003022 005 2014-12-17 008 2014 bl uuuu u00u1 u #d 100 1 $aALVAREZ-PIZARRO, J. C. 245 $aPlasma membrane H+-ATPase in sorghum roots as affected by potassium deficiency and nitrogen sources. 260 $c2014 520 $aAbstract: We studied the influence of inorganic nitrogen sources (NO3- or NH4+) and potassium deficiency on expression and activity of plasma membrane (PM) H+-ATPase in sorghum roots. After 15 d of cultivation at 0.2 mM K+, the plants were transferred to solutions lacking K+ for 2 d. Then, K+ depletion assays were performed in the presence or absence of vanadate. Further, PMs from K+-starved roots were extracted and used for the kinetic characterization of ATP hydrolytic activity and the immunodetection of PM H+-ATPase. Two major genes coding PM H+-ATPase (SBA1 and SBA2) were analyzed by real-time PCR. PM H+-ATPase exhibited a higher Vmax and Km in NH4+-fed roots compared with NO3--fed roots. The optimum pH of the enzyme was slightly lower in NO3- -fed roots than in NH4+ -fed roots. The vanadate sensitivity was similar. The expressions of SBA1 and SBA2 were increased in roots grown under NH4+. Concomitantly, an increased content of the enzyme in PM was observed. The initial rate of K+ uptake did not differ between plants grown with NO3- or NH4+, but it was significantly reduced by vanadate in NH4+-grown plants. 650 $aAmmonium nitrate 650 $aNitrate fertilizers 650 $aPotassium 650 $aFertilizante nitrogenado 650 $aPotássio 650 $aSorghum Bicolor 650 $aSorgo 653 $aNitrato de Amonia 700 1 $aGOMES FILHO, E. 700 1 $aPRISCO, J. T. 700 1 $aGROSSI DE SA, M. F. 700 1 $aOLIVEIRA NETO, O. B. de 700 1 $aFRAGOSO, R. da R. 773 $tBiologia Plantarum$gv. 58, n. 3, p. 507-514, 2014.
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